![]() ![]() For this reason, no donor-dependent factors other than sex, number of injected cells, and human leukocyte antigen (HLA) matching that affect transplant treatment outcomes have been reported.ĭoes cord blood, which is 0 years old at the time of transplantation, change in age over time in the patient’s bone marrow? If so, what factors affect this, and can this be evaluated by DNA methylation? The purpose of our study is to clarify the extent to which the DNAm age differs from the chronological cord blood age in the patient’s body and to clarify whether there is a relationship between the occurrence of graft-versus-host disease (GVHD), a post-transplant complication, the recipient background, and DNAm age. Therefore, unlike other donor sources, the donor age at the time of transplantation is unified to 0 years. In contrast to that of donor sources such as BM or PBSCs, the DNA methylation age of cord blood nucleated cells has been shown to be 0 years 5. To the best of our knowledge, DNAm age analysis in cord blood transplant cases has not been performed to date. Thus, it has been shown that the DNAm age of donor cells after transplantation may differ depending on the collection method.Ĭord blood is a widely used alternative transplant source. ![]() In a DNAm age analysis by Horvath clock, PBSC transplantation primed by granulocyte colony-stimulating factor (G-CSF) significantly reduced the DNAm age of the donor cells compared to the chronological age of the donor but tended to increase cell age in BM transplant donors 11. The DNA methylation (DNAm) age of the donor from bone marrow (BM) or peripheral blood stem cells (PBSCs) after transplantation is not affected by the recipient’s age and is reported to be approximately equivalent to the donor’s age. ![]() This new method has revealed that cellular aging is accelerated by various diseases, compared to that in healthy people 6, 7.ĭNA methylation analysis of donor cell age in patients with hematopoietic stem cell transplantation has been reported 8, 9, 10. These findings in stem cell biology are indispensable to develop better regenerative medicine procedures.Ĭhanges in telomere length and epigenetic DNA alterations have allowed for analysis of the aging mechanism of donor cells after transplantation 1, 2 however, in the past 10 years, DNA methylation sites closely related to temporal age have been revealed, making it possible to estimate age from DNA 3, 5. It provides an important step in understanding how stem cells age 1, 2, 3, 4. These transplants offer a window into how donor cells age in the recipient’s body after transplantation and whether stem cell aging is affected by changes in their environment. The results shed light on the importance of the mismatch between cord blood stem cells and donor environmental factors in stem cell aging.Īllogeneic hematopoietic stem cell transplantation is a very powerful treatment that can cure blood diseases. However, this analysis revealed that transplanting 0-year-old cord blood into a recipient resulted in cells aging more than twice as quickly as the elapsed time. The characteristics of patients and transplant procedures were not associated with AgeAccel in this analysis, nor were they associated with the development of graft-versus-host disease. ![]() When comparing the mean values of AgeAccel in cord blood transplant cases and controls, the values were significantly higher in cord blood transplant cases. The median chronological age (the time elapsed from the date of the cord blood transplant to the day the sample was taken for DNAm analysis) of donor cells was 4.0 years (0.2–15.0 years), while the median DNAm age was 10.0 years (1.3–30.3 years), and the ratio of DNAm age to chronological age (AgeAccel) was 2.7 (1.2–8.2). We performed DNA methylation (DNAm) age analysis to measure the age of cells using post-transplant peripheral blood in 50 cases of cord blood transplantation. However, it is unclear how cord blood cells, which are 0 years old, age in the recipient’s body after allogeneic transplantation. Cord blood stem cell transplantation is an important alternative for patients needing hematopoietic stem cell transplantation. ![]()
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